Flowering and Runnering of Seasonal Strawberry under Different Photoperiods Are Affected by Intensity of Supplemental or Night-Interrupting Blue Light.

The strawberry (Fragaria × ananassa Duch.) "Sulhyang" is a typical seasonal flowering (SF) strawberry that produces flower buds in day lengths shorter than a critical limit (variable, but often defined as <12 h). There is a trade-off between photoperiod-controlled flowering and gibberellin (GA) signaling pathway-mediated runnering. Some related genes (such as CO, FT1, SOC1, and TFL1) participating in light signaling and circadian rhythm in plants are altered under blue light (BL). Sugars for flowering and runnering are mainly produced by photosynthetic carbon assimilation. The intensity of light could affect photosynthesis, thereby regulating flowering and runnering. Here, we investigated the effect of the intensity of supplemental blue light (S-BL) or night-interrupting blue light (NI-BL) in photoperiodic flowering and runnering regulation by applying 4 h of S-BL or NI-BL with either 0, 10, 20, 30, or 40 µmol·m-2·s-1 photosynthetic photon flux density (PPFD) in a 10 h short-day (SD10) (SD10 + S-BL4 or + NI-BL4 (0, 10, 20, 30, or 40)) or 14 h long-day (LD14) conditions (LD14 + S-BL4 or + NI-BL4 (0, 10, 20, 30, or 40)). Approximately 45 days after the photoperiodic light treatment, generally, whether S-BL or NI-BL, BL (20) was the most promotive in runnering, leading to more runners in both the LD and SD conditions. For flowering, except the treatment LD14 + S-BL, BL (20) was still the key light, either from BL (20) or BL (40), promoting flowering, especially when BL acted as the night-interrupting light, regardless of the photoperiod. At the harvest stage, larger numbers of inflorescences and runners were observed in the LD14 + NI-BL4 treatment, and the most were observed in the LD14 + NI-BL (20). Moreover, the SD10 + NI-BL4 was slightly inferior to the LD14 + NI-BL4 in increasing the numbers of inflorescences and runners, but it caused earlier flowering. Additionally, the circadian rhythm expression of flowering-related genes was affected differently by the S-BL and NI-BL. After the application of BL in LD conditions, the expression of an LD-specific floral activator FaFT1 was stimulated, while that of a flowering suppressor FaTFL1 was inhibited, resetting the balance of expression between these two opposite flowering regulators. The SD runnering was caused by BL in non-runnering SD conditions associated with the stimulation of two key genes that regulate runner formation in the GA pathway, FaGRAS32 and FaGA20ox4. In addition, the positive effects of BL on enhancing photosynthesis and carbohydrate production also provided an abundant energy supply for the flowering and runnering processes.

Shift in the Light Quality of Night Interruption Affects Flowering and Morphogenesis of Petunia hybrida.

Petunia hybrida Hort. "Easy Wave Pink", a qualitative long-day plant (LDP), was investigated to study the effects of the night interruption light (NIL) provided by light-emitting diodes (LEDs) quality shifting on the morphogenesis, blooming, and transcription of photoreceptor genes. Plants were grown in a closed-type plant factory employing white (W) LEDs at an intensity of 180 µmol·m-2·s-1 PPFD provided for short day (SD, 10 h light, 14 h dark), long day (LD, 16 h light, 8 h dark), or SD with 4 h night interruption (NI) with LEDs at an intensity of 10 µmol·m-2·s-1 PPFD. The NIL quality was shifted from one light spectrum to another after the first 2 h of NI. Light treatments consisting of all possible pairings of W, far-red (Fr), red (R), and blue (B) light were tested. The SD and LD were referenced as the control, while 12 NI treatments involved altering LED NIL qualities, as follows: from R to B (NI-RB), from B to R (NI-BR), from Fr to R (NI-FrR), from R to Fr (NI-RFr), from Fr to B (NI-FrB), from B to Fr (NI-BFr), from B to W (NI-BW), from W to B (NI-WB), from W to Fr (NI-WFr), from Fr to W (NI-FrW), from W to R (NI-WR), and from R to W (NI-RW). The NI-RFr resulted in the longest shoots, while the NI-WR and NI-RW resulted in the shortest shoots. NI-WR, NI-RW, NI-BW, NI-WB, NI-RFr, NI-RB, NI-BR, and LD all exhibited flowering. High-level expressions of photoreceptor genes were confirmed in the NI-RFr, NI-FrR, NI-BFr, NI-RW, and NI-WR treatments. Morphogenesis and blooming were both impacted by the photoperiod. The first NIL had no effects on the flowering or the morphogenesis, but the second NIL had a profound impact on both.

Exploring the potential: Can arsenic (As) resistant silicate-solubilizing bacteria manage the dual effects of silicon on As accumulation in rice?

Rice (Oryza sativa L.) cultivation in regions marked by elevated arsenic (As) concentrations poses significant health concerns due to As uptake by the plant and its subsequent entry into the human food chain. With rice serving as a staple crop for a substantial share of the global population, addressing this issue is critical for food security. In flooded paddy soils, where As availability is pronounced, innovative strategies to reduce As uptake and enhance agricultural sustainability are mandatory. Silicon (Si) and Si nanoparticles have emerged as potential candidates to mitigate As accumulation in rice. However, their effects on As uptake exhibit complexity, influenced by initial Si levels in the soil and the amount of Si introduced through fertilization. While low Si additions may inadvertently increase As uptake, higher Si concentrations may alleviate As uptake and toxicity. The interplay among existing Si and As availability, Si supplementation, and soil biogeochemistry collectively shapes the outcome. Adding water-soluble Si fertilizers (e.g., Na2SiO3 and K2SiO3) has demonstrated efficacy in mitigating As toxicity stress in rice. Nonetheless, the expense associated with these fertilizers underscores the necessity for low cost innovative solutions. Silicate-solubilizing bacteria (SSB) resilient to As hold promise by enhancing Si availability by accelerating mineral dissolution within the rhizosphere, thereby regulating the Si biogeochemical cycle in paddy soils. Promoting SSB could make cost-effective Si sources more soluble and, consequently, managing the intricate interplay of Si's dual effects on As accumulation in rice. This review paper offers a comprehensive exploration of Si's nuanced role in modulating As uptake by rice, emphasizing the potential synergy between As-resistant SSB and Si availability enhancement. By shedding light on this interplay, we aspire to shed light on an innovative attempt for reducing As accumulation in rice while advancing agricultural sustainability.

Difference between Day and Night Temperature (DIF) and Light Intensity Affect Growth and Photosynthetic Characteristics of Panax ginseng Meyer Sprouts.

Panax ginseng sprouts (PGS) have the advantage of requiring short-term cultivation while maintaining higher ginsenoside contents than traditional ginseng seedlings. It is feasible to improve their yield capacity by manipulating physical factors such as temperature and light. This study therefore investigated the effects of the DIF (difference between day and night temperature) and LI (light intensity) on the growth and photosynthetic characteristics of PGS. To this end, four DIF treatments (18/22 °C, 20/20 °C, 22/22 °C, 22/18 °C), corresponding to two LI regimes (20 PPFD, 200 PPFD), were applied on one-year-old ginseng rootlets in closed-type plant production systems (CPPSs). The PGS had distinctly different responses to the eight treatments. In particular, we found that negative DIF considerably hampered the growth and development of roots, shoots, leaves, and photosynthesis, regardless of the LI considered. The PGS treated with 20/20 °C combined with 20 PPFD displayed the best root growth, shoot development, leaf area, as well as optimal photosynthetic ability. On the other hand, we further showed that the root growth rate was positively correlated with the stem diameter, leaf traits, and photosynthetic ability, whereas it was negatively correlated with the petiole length, stem length, and shoot length. Collectively, 20/20 °C combined with 20 PPFD was the optimal condition in the current study, and may be regarded as a successful strategy for large-scale productions of PGS.

Top and Side Lighting Induce Morphophysiological Improvements in Korean Ginseng Sprouts (Panax ginseng C.A. Meyer) Grown from One-Year-Old Roots.

Nowadays, not only the roots, but also leaves and flowers of ginseng are increasingly popular ingredients in supplements for healthcare products and traditional medicine. The cultivation of the shade-loving crop, ginseng, is very demanding in terms of the light environment. Along with the intensity and duration, light direction is another important factor in regulating plant morphophysiology. In the current study, three lighting directions-top (T), side (S), or top + side (TS)-with an intensity of 30 ± 5 µmol·m-2·s-1 photosynthetic photon flux density (PPFD) were employed. Generally, compared with the single T lighting, the composite lighting direction, TS, was more effective in shaping the ginseng with improved characteristics, including shortened, thick shoots; enlarged, thick leaves; more leaf trichomes; earlier flower bud formation; and enhanced photosynthesis. The single S light resulted in the worst growth parameters and strongly inhibited the flower bud formation, leading to the latest flower bud observation. Additionally, the S lighting acted as a positive factor in increasing the leaf thickness and number of trichomes on the leaf adaxial surface. However, the participation of the T lighting weakened these traits. Overall, the TS lighting was the optimal direction for improving the growth and development traits in ginseng. This preliminary research may provide new ideas and orientations in ginseng cultivation lodging resistance and improving the supply of ginseng roots, leaves, and flowers to the market.

Arsenic (As) resistant bacteria with multiple plant growth-promoting traits: Potential to alleviate As toxicity and accumulation in rice.

A currently serious agronomic concern for paddy soils is arsenic (As) contamination. Paddy soils are mostly utilized for rice cultivation. Arsenite (As(III)) is prevalent in paddy soils, and its high mobility and toxicity make As uptake by rice substantially greater than that by other food crops. Globally, interest has increased towards using As-resistant plant growth-promoting bacteria (PGPB) to improve plant metal tolerance, promote plant growth, and immobilize As to prevent its uptake and accumulation in the edible parts of rice as much as possible. This review focuses on the As-resistant PGPB characteristics influencing rice growth and the mechanisms by which they function to alleviate As toxicity stress in rice plants. Several recent examples of mechanisms responsible for decreasing the availability of As to rice and coping with As stresses facilitated by the PGPB with multiple PGP traits (e.g., phosphate and silicate solubilization, the production of 1-aminocyclopropane-1-carboxylate deaminase, phytohormones, and siderophore, N2 fixation, sulfate reduction, the biosorption, bioaccumulation, methylation, and volatilization of As, and arsenite oxidation) are also reviewed. In addition, future research needs about the application of As-resistant PGPB with PGP traits to mitigate As accumulation in rice plants are described.

Blue Light Supplemented at Intervals in Long-Day Conditions Intervenes in Photoperiodic Flowering, Photosynthesis, and Antioxidant Properties in Chrysanthemums.

The flowering of chrysanthemum (Chrysanthemum morifolium Ramat.), inhibited by long-day lighting, can be reversed with a short period of low supplemental blue light (S-BL). Both flowering and the reactive oxygen species (ROS) scavenging processes are primarily driven by sugars created by photosynthetic carbon assimilation. In addition, the antioxidant ability potentially affects flowering in photoperiod- and/or circadian rhythm-dependent manners. This indicates that there is an interactive relationship among blue (B) light, photosynthetic efficiency, sugar accumulation, and antioxidant ability in flowering regulation. Here, 4 h of 30 µmol·m-2·s-1 photosynthetic photon flux density (PPFD) S-BL was applied at the end of a 13-h long-day period (LD13 + 4B) at different intervals during 60 days of experimental duration. The five experimental groups were named according to the actual number of days of S-BL and their intervals: applied once every day, "60 days-(LD13 + 4B) (100.0%)"; once every other day, "30 days-(LD13 + 4B) (50.0%)"; once every three days, "15 days-(LD13 + 4B) (25.0%)"; once every five days, "10 days-(LD13 + 4B) (16.7%)"; and once every seven days, "7 days-(LD13 + 4B) (11.7%)". Two non-S-BL control groups were also included: 60 10-h short days (60 days-SD10) and 13-h long days (60 days-LD13). At the harvest stage, varying degrees of flowering were observed except in "60 days-LD13" and "7 days-(LD13 + 4B) (11.7%)". The number of flowers increased and the flower buds appeared earlier as the proportion of S-BL days increased in LD13 conditions, although the "60 days-SD10" gave the earliest flowering. The proportion of initial, pivotal, and optimal flowering was 16.7% ("10 days-(LD13 + 4B)"), 50.0% ("30 days-(LD13 + 4B)"), and 100.0% ("60 days-(LD13 + 4B)"), respectively. Meanwhile, a series of physiological parameters such as the production of enzymatic or non-enzymatic antioxidants, chlorophyll content, photosynthetic efficiency, enzyme activities, and carbohydrate accumulation were significantly improved by "30 days-(LD13 + 4B) (50.0%)" as a turning point until the peaks appeared in "60 days-(LD13 + 4B) (100.0%)", as well as the expression of florigenic or anti-florigenic and some antioxidant-synthetic genes. Furthermore, the results of principal component analysis (PCA) indicated that S-BL days positively regulated flowering, photosynthesis, carbohydrate accumulation, and antioxidant production. In aggregate, the pivotal and optimal proportions of S-BL days to reconcile the relationship among flowering, photosynthetic carbon assimilation, and antioxidant ability were 50.0% and 100.0%, respectively. However, there are still significant gaps to be filled in order to determine the specific involvement of blue light and antioxidant abilities in flowering regulation.

Silicon Supplementation Alleviates Adverse Effects of Ammonium on Ssamchoo Grown in Home Cultivation System.

Ssamchoo is recently attracting attention as a household hydroponic vegetable in Korea. It has a refreshing texture and a rich content of vitamins and fiber. Ssamchoo with a wide leaf area is suitable for traditional ssam or vegetable wraps, as well as a vegetable for salads; thus, it can be used in a variety of dishes. However, Ssamchoo plants responds sensitively to the nutrient solution, and it is often difficult to secure sufficient leaf area and robust growth using a commercial nutrient solution for leafy vegetables. This study consisted of three experiments conducted to develop the nutrient solution for Ssamchoo grown in a newly developed home hydroponic cultivation system using light-emitting diodes as the sole source of light. In the first experiment, growth and development of Ssamchoo in a representative commercial nutrient solution, Peters Professional (20-20-20, The Scotts Co., Marysville, OH, USA), was compared with laboratory-prepared nutrient solutions, GNU1 and GNU2. As a result, the Ssamchoo grown in Peters Professional had a high NH4+ content in the tissue, leaf yellowing, darkened root color, and suppressed root hair development. In addition, adverse effects of ammonium such as low fresh weight and shorter shoot length were observed. In the second experiment, Peters Professional was excluded, and the ratio of NO3- to NH4+ in the GNU1 and GNU2 nutrient solutions was set to four levels each (100:0, 83.3:16.7, 66.7:33.3, and 50:50). As a result, the fresh weights of 83.3:16.7 and 66.7:33.3 were the greatest, and the leaf color was a healthy green. However, at 100:0 and 50:50 NO3-/NH4+ ratios, the fresh weight was low, and leaf yellowing, tip burn, and leaf burn appeared. The nutrient solution with a 83.3:16.7 NO3-- to-NH4+ ratio, which gave the greatest fresh weight in the second experiment, was chosen as the control, while the solution with a 50:50 NO3-/NH4+ ratio with a lower nitrate content among the two unfavorable treatments was selected as a treatment group for the next experiment. In the third experiment, NH4+ was partially replaced with urea to make four different ratios of NO3- to NH4+ to urea (83:17:0, 50:50:0, 50:25:25, and 50:0:50) in combination with two levels of Si (0 and 10.7 mmol·L-1 Si). The greatest fresh weight was obtained in the treatment in which the NO3-/NH4+/urea ratio was 50:25:25. In particular, when Si was added to the solution, there was no decrease in the number of leaves, and plants with the greatest fresh weight, chlorophyll content, and leaf area were obtained. The number of leaves and leaf area are important indicators of high productivity since the Ssamchoo is used in ssam dishes. It can be concluded that a solution with a NO3-/NH4+/urea ratio of 50:25:25 and supplemented with 10.7 mmol·L-1 Si is the most suitable nutrient solution for growing Ssamchoo in the home hydroponic system developed.

Synergistic Effects of Silicon and Preservative on Promoting Postharvest Performance of Cut Flowers of Peony (Paeonia lactiflora Pall.).

As a commercial high-grade cut flower, the marketability of herbaceous peony (Paeonia lactiflora Pall.) is limited by its short vase life in water. Si (silicon) is an alternative to improve the postharvest life of cut flowers. However, the effects of the combined application of Si and preservatives on the postharvest performance of cut peony flowers are unknown. In this study, the effects of a Si application and a preservative alone and collegial on the longevity of the vase life, water loss, antioxidant defense system, and stock carbohydrates level of cut flowers of three peony cultivars were investigated. It was observed that Si effectively prolonged the vase life, while the preservative alone, to a lesser extent, but markedly induced an early flowering and a greater flower diameter (flower open degree). The simultaneous use of Si and the preservatives not only showed larger flowers, but also improved the postharvest performance as characterized by an extended vase life and delayed the water loss. In addition, the Si supplementation dramatically intensified the antioxidant defense system (ameliorated antioxidant enzymes and alleviated ROS accumulation) in petals but did not increase the stock carbohydrates (starch and soluble sugars) levels, as compared to the treatment with the preservative alone. We show that a Si supplementation to a preservative is highly recommended for a large-scale use to promote the postharvest performance and competitiveness of marketed cut flowers.

The flowering of SDP chrysanthemum in response to intensity of supplemental or night-interruptional blue light is modulated by both photosynthetic carbon assimilation and photoreceptor-mediated regulation.

The photoreceptor-mediated photoperiodic sensitivity determines the obligate short-day flowering in chrysanthemum (Chrysanthemum morifolium Ramat.) when the night length is longer than a critical minimum, otherwise, flowering is effectively inhibited. The reversal of this inhibition by subsequent exposure to a short period of supplemental (S) or night-interruptional (NI) blue (B) light (S-B; NI-B) indicates the involvement of B light-received photoreceptors in the flowering response. Flowering is mainly powered by sugars produced through photosynthetic carbon assimilation. Thus, the light intensity can be involved in flowering regulation by affecting photosynthesis. Here, it is elucidated that the intensity of S-B or NI-B in photoperiodic flowering regulation of chrysanthemums by applying 4-h of S-B or NI-B with either 0, 10, 20, 30, or 40 µmol·m-2·s-1 photosynthetic photon flux density (PPFD) in a 10-h short-day (SD10) [SD10 + 4B or + NI-4B (0, 10, 20, 30, or 40)] or 13-h long-day (LD13) condition [LD13 + 4B or + NI-4B (0, 10, 20, 30, or 40)] provided by 300 ± 5 µmol·m-2·s-1 PPFD white (W) LEDs. After 60 days of photoperiodic light treatments other than the LD13 and LD13 + NI-4B (40), flowering with varying degrees was observed, although the SD10 gave the earliest flowering. And the LD13 + 4B (30) produced the greatest number of flowers. The flowering pattern in response to the intensity of S-B or NI-B was consistent as it was gradually promoted from 10 to 30 µmol m-2 s-1 PPFD and inhibited by 40B regardless of the photoperiod. In SD conditions, the same intensity of S-B and NI-B did not significantly affect flowering, while differential flowering inhibition was observed with any intensity of NI-B in LDs. Furthermore, the 30 µmol·m-2·s-1 PPFD of S-B or NI-B up-regulated the expression of floral meristem identity or florigen genes, as well as the chlorophyll content, photosynthetic efficiency, and carbohydrate accumulation. The 40B also promoted these physiological traits but led to the unbalanced expression of florigen or anti-florigen genes. Overall, the photoperiodic flowering in response to the intensity of S-B or NI-B of the SDP chrysanthemum suggests the co-regulation of photosynthetic carbon assimilation and differential photoreceptor-mediated control.

Alleviation of Ammonium Toxicity in Salvia splendens 'Vista Red' with Silicon Supplementation.

Ammonium (NH4+) toxicity seriously hampers the yield and quality of salvia plants because most varieties or sub-species are highly sensitive to NH4+. Silicon (Si) is an alternative that is used to minimize these disturbances and maintain better growth under NH4+ toxicity. Nevertheless, the mitigatory effects of Si on NH4+-stressed salvia are unknown. Therefore, this study was carried out to determine how Si assists to alleviate the NH4+ toxicity degree in salvia. To this end, salvia plants were cultivated in a controlled environment supplied with a constant N (nitrogen) level (13 meq·L-1) in the form of three NH4+:NO3- ratios (0:100, 50:50, 100:0), each with (1.0 meq·L-1) or without Si. Physiological disorders and typical NH4+ toxicity symptoms, as well as interrupted photosynthesis, were observed in the 100% NH4+-treated plants. Furthermore, cation uptake inhibition and oxidative damage were also imposed by the 100% NH4+ supply. In contrast, in the presence of Si, the NH4+ toxicity degree was attenuated and plant growth was ensured. Accordingly, the NH4+ toxicity appearance ratio decreased significantly. Furthermore, Si-treated plants showed an ameliorated photosynthetic ability, elevated internal K and Ca levels, and enhanced antioxidative capacity, as reflected by improved major antioxidant enzyme activities, as well as diminished accumulation of ROS (reactive oxygen species) and MDA (malondialdehyde). Our findings enlightened the agronomic importance of additional Si to nutrient solutions, especially pertaining to bedding plants at risk of NH4+ toxicity.

Iron Source and Medium pH Affect Nutrient Uptake and Pigment Content in Petunia hybrida 'Madness Red' Cultured In Vitro.

Deficiency or excess of iron (Fe) and improper medium pH will inhibit the growth and development of plants, reduce the transfer and utilization of energy from the root to the leaf, and affect the utilization efficiency of inorganic nutrients. The most common symptom of Fe deficiency in plants is chlorosis of the young leaves. In this study, the effects of the iron source, in combination with the medium pH, on plant growth and development, plant pigment synthesis, and nutrient uptake in a model plant Petunia hybrida cultured in vitro were investigated. Iron sulfate (FeSO4·7H2O) or iron chelated with ethylenediaminetetraacetic acid (Fe-EDTA) were supplemented to the MNS (a multipurpose nutrient solution) medium at a concentration of 2.78 mg·L-1 Fe, and the treatment without any Fe was used as the control. The pH of the agar-solidified medium was adjusted to either 4.70, 5.70, or 6.70 before autoclaving. The experiment was carried out in an environmentally controlled culture room with a temperature of 24 °C with 100 µmol·m-2·s-1 photosynthetic photon flux density (PPFD) supplied by white light emitting diodes (LEDs) during a photoperiod of 16 h a day, 18 °C for 8 h a day in the dark, and 70% relative humidity. Regardless of the Fe source including the control, the greatest number of leaves was observed at pH 4.70. However, the greatest lengths of the leaf and root were observed in the treatment with Fe-EDTA combined with pH 5.70. The contents of the chlorophyll, carotenoid, and anthocyanin decreased with increasing medium pH, and contents of these plant pigments were positively correlated with the leaf color. The highest soluble protein content and activities of APX and CAT were observed in the Fe-EDTA under pH 5.70. However, the GPX activity was the highest in the control under pH 4.70. In addition, the highest contents of ammonium (NH4+) and nitrate (NO3-) were measured in the FeSO4-4.7 and EDTA-5.7, respectively. More than that, the treatment of Fe-EDTA combined with pH 5.70 (EDTA-5.7) enhanced nutrient absorption, as proven by the highest tissue contents of P, K, Ca, Mg, Fe, and Mn. The genes' ferric reduction oxidase 1 and 8 (PhFRO1 and PhFRO8), iron-regulated transporter 1 (PhIRT1), nitrate transporter 2.5 (PhNRT2.5), and deoxyhypusine synthase (PhDHS) were expressed at the highest levels in this treatment as well. In the treatment of EDTA-5.7, the reduction and transport of chelated iron in P. hybrida leaves were enhanced, which also affected the transport of nitrate and catalyzed chlorophyll level in leaves. In conclusion, when the medium pH was adjusted to 5.70, supplementation of chelated Fe-EDTA was more conducive to promoting the growth and development of, and absorption of mineral nutrients by, the plant and the expression of related genes in the leaves.

Low-Intensity Blue Light Supplemented during Photoperiod in Controlled Environment Induces Flowering and Antioxidant Production in Kalanchoe.

Kalanchoe (Kalanchoe blossfeldiana) is a qualitative short-day plant with a high aesthetic value. When the night length is less than a specified cultivar-dependent critical value, however, it does not develop flowers. This study investigated the effects of low-intensity supplementary or night interrupting (NI) blue (B) light on the plant performance and flower induction in kalanchoe 'Rudak'. During the photoperiod in a closed-type plant factory with day/night temperatures of 23 °C/18 °C, white (W) LEDs were utilized to produce a photosynthetic photon flux density (PPFD) of 300 µmol m-2 s-1, and B LEDs were used to give supplementary/NI light at a PPFD of 10 µmol m-2 s-1. The control plants were exposed to a 10-h short day (SD, positive control) or a 13-h long day (LD, negative control) treatment without any B light. The B light was used for 4 h either (1) to supplement the W LEDs at the end of the SD (SD + 4B) and LD (LD + 4B), or (2) to provide night interruption (NI) in the SD (SD + NI-4B) and LD (LD + NI-4B). The LD + 4B and LD + NI-4B significantly enhanced plant growth and development, followed by the SD + 4B and SD + NI-4B treatments. In addition, the photosynthesis, physiological parameters, and activity of antioxidant systems were improved in those treatments. Except in the LD and LD + NI-4B, all plants flowered. It is noteworthy that kalanchoe 'Rudak' flowered in the LD + 4B treatment and induced the greatest number of flowers, followed by SD + NI-4B and SD + 4B. Plants grown in the LD + 4B treatment had the highest expression levels of certain monitored genes related to flowering. The results indicate that a 4-h supplementation of B light during the photoperiod in both the SD and LD treatments increased flower bud formation, promoted flowering, and enhanced plant performance. Kalanchoe 'Rudak' flowered especially well in the LD + 4B, presenting a possibility of practically inducing flowering in long-day seasons with B light application.

Foliar Silicon Spray before Summer Cutting Propagation Enhances Resistance to Powdery Mildew of Daughter Plants.

Silicon (Si) has beneficial effects on not only plant growth but also against biotic and abiotic stresses. However, a few studies focus on how Si application helps strawberry (Fragaria × ananassa Duch.) resist powdery mildew. The aim of this work was to find out the optimal Si application method before cutting propagation to enhance the resistance to powdery mildew in strawberry "daughter" plants. Naturally infected "mother" plants of 'Sulhyang', 'Maehyang', and 'Kuemsil' strawberries were supplied with Si. Potassium silicate (K2SiO3) at a final concentration of 75 mg·L-1 Si was either added to the medium for drenching or sprayed to the leaves of the "mother" or "daughter" plant, or soluble Si fertilizer was used to dress the "mother" plant. The Si application significantly increased the shoot fresh weight of the "daughter" plants. Supplemental Si also increased the contents of phosphorus (P), potassium (K), and magnesium (Mg). In addition, the Si treatment decreased the damage of powdery mildew by increased level of proline content and suppressive reactive oxygen species. After applying Si, the length and density of hyphae on the leaf surface decreased. In addition, the infected area of "daughter" plant leaves covered with powdery mildew decreased. This study also demonstrated that Si increased the expression of resistance-gene and decreased the expression of susceptibility-gene of strawberry. Overall, Si application promoted the growth of the "daughter" plants regardless of the application method. Direct foliar Si spray to the "daughter" plants before cutting propagation is recommended to increase their resistance to powdery mildew.

Drenched Silicon Suppresses Disease and Insect Pests in Coffee Plant Grown in Controlled Environment by Improving Physiology and Upregulating Defense Genes.

Plant disease and insect pests are major limiting factors that reduce crop production worldwide. The ornamental indoor cultivation cash crop dwarf coffee Punica arabica 'Pacas' is also troubled by these issues. Silicon (Si) is one of the most abundant elements in the lithosphere and positively impacts plant health by effectively mitigating biotic and abiotic stresses. Several studies have shown that Si activates plant defense systems, although the specific nature of the involvement of Si in biochemical processes that lead to resistance is unclear. In our study, Si significantly promoted the growth and development of dwarf coffee seedlings grown in plant growth chambers. More than that, through natural infection, Si suppressed disease and insect pests by improving physiology (e.g., the strong development of the internal structures of roots, stems, and leaves; higher photosynthetic efficiency; more abundant organic matter accumulation; the promotion of root activity; the efficient absorption and transfer of mineral elements; and various activated enzymes) and up-regulating defense genes (CaERFTF11 and CaERF13). Overall, in agriculture, Si may potentially contribute to global food security and safety by assisting in the creation of enhanced crop types with optimal production as well by mitigating plant disease and insect pests. In this sense, Si is a sustainable alternative in agricultural production.

Lighting from Top and Side Enhances Photosynthesis and Plant Performance by Improving Light Usage Efficiency.

Light is a critical environmental factor that influences plant growth and development, ranging from seed germination to flowering and fruiting. This study was carried out to explore how the optimal combination of various lighting directions increases the light usage efficiency and influences the plant morphophysiology, by investigating the plant growth parameters, leaf anatomy, epidermal morphology, stomatal properties, chlorophyll content, key physiological changes, and correlated gene expressions. In closed-type plant growth chambers, rooted cuttings of two chrysanthemum (Chrysanthemum morifolium Ramat.) cultivars, "Pearl Egg" and "Gaya Glory", were subjected to a 10-h photoperiod with 600 µmol∙m-2·s-1 photosynthetic photon flux density (PPFD) provided by light-emitting diodes (LEDs) in each light-direction combination (top (1/1) (T), top (1/2) + side (1/2) (TS), top (1/2) + bottom (1/2) (TB), side (1/2) + bottom (1/2) (SB), and top (1/3) + side (1/3) + bottom (1/3) (TSB)). The TS lighting significantly enhanced the morphophysiological performance, compared to the other lighting direction combinations. Notably, the excellent branch formation and earlier flowering were induced by the TS lighting in both "Pearl Egg" and "Gaya Glory" plants.

Root GS and NADH-GDH Play Important Roles in Enhancing the Ammonium Tolerance in Three Bedding Plants.

Ammonium is a paradoxical nutrient because it is more metabolically efficient than nitrate, but also causes plant stresses in excess, i.e., ammonium toxicity. Current knowledge indicates that ammonium tolerance is species-specific and related to the ammonium assimilation enzyme activities. However, the mechanisms underlying the ammonium tolerance in bedding plants remain to be elucidated. The study described herein explores the primary traits contributing to the ammonium tolerance in three bedding plants. Three NH4+:NO3- ratios (0:100, 50:50, 100:0) were supplied to salvia, petunia, and ageratum. We determined that they possessed distinct ammonium tolerances: salvia and petunia were, respectively, extremely sensitive and moderately sensitive to high NH4+ concentrations, whereas ageratum was tolerant to NH4+, as characterized by the responses of the shoot and root growth, photosynthetic capacity, and nitrogen (amino acid and soluble protein)-carbohydrate (starch) distributions. An analysis of the major nitrogen assimilation enzymes showed that the root GS (glutamine synthetase) and NADH-GDH (glutamate dehydrogenase) activities in ageratum exhibited a dose-response relationship (reinforced by 25.24% and 6.64%, respectively) as the NH4+ level was raised from 50% to 100%; but both enzyme activities were significantly diminished in salvia. Besides, negligible changes of GS activities monitored in leaves revealed that only the root GS and NADH-GDH underpin the ammonium tolerances of the three bedding plants.

Seleno-Amino Acids in Vegetables: A Review of Their Forms and Metabolism.

Seleno-amino acids are safe, health-promoting compounds for humans. Numerous studies have focused on the forms and metabolism of seleno-amino acids in vegetables. Based on research progress on seleno-amino acids, we provide insights into the production of selenium-enriched vegetables with high seleno-amino acids contents. To ensure safe and effective intake of selenium, several issues need to be addressed, including (1) how to improve the accumulation of seleno-amino acids and (2) how to control the total selenium and seleno-amino acids contents in vegetables. The combined use of plant factories with artificial lighting and multiple analytical technologies may help to resolve these issues. Moreover, we propose a Precise Control of Selenium Content production system, which has the potential to produce vegetables with specified amounts of selenium and high proportions of seleno-amino acids.

Side Lighting Enhances Morphophysiology by Inducing More Branching and Flowering in Chrysanthemum Grown in Controlled Environment.

Light is one of the most important factors that influence plant growth and development. This study was conducted to examine how lighting direction affects plant morphophysiology by investigating plant growth parameters, leaf anatomy, epidermal cell elongation, stomatal properties, chloroplast arrangement, and physiological changes. In closed-type plant factory units, the rooted cuttings of two chrysanthemum (Chrysanthemum morifolium Ramat.) cultivars, 'Gaya Glory' and 'Pearl Egg', were subjected to a 10 h photoperiod with a 300 µmol∙m-2∙s-1 photosynthetic photon flux density (PPFD) provided by light-emitting diodes (LEDs) from three directions relative to the plant including the top, side, and bottom. Compared to the top or bottom lighting, the side lighting greatly enhanced the plant growth, improved the leaf internal structure and chloroplast arrangement, induced small stomata with a higher density, and promoted stomatal opening, which is associated with an increased stomatal conductance and photosynthetic efficiency. It is worth noting that the side lighting significantly enhanced the induction of branching and flowering for both cultivars., The plants grown with side lighting consistently exhibited the greatest physiological performance. We conclude that the lighting direction had a profound effect on the morphophysiological characteristics of chrysanthemum, and that side lighting dramatically promoted their growth and development, especially in their branching and flowering.